An advanced fragment analysis-based individualized subtype classification of pediatric acute lymphoblastic leukemia
نویسندگان
چکیده
Pediatric acute lymphoblastic leukemia (ALL) is the most common neoplasm and one of the primary causes of death in children. Its treatment is highly dependent on the correct classification of subtype. Previously, we developed a microarray-based subtype classifier based on the relative expression levels of 62 marker genes, which can predict 7 different ALL subtypes with an accuracy as high as 97% in completely independent samples. Because the classifier is based on gene expression rank values rather than actual values, the classifier enables an individualized diagnosis, without the need to reference the background distribution of the marker genes in a large number of other samples, and also enables cross platform application. Here, we demonstrate that the classifier can be extended from a microarray-based technology to a multiplex qPCR-based technology using the same set of marker genes as the advanced fragment analysis (AFA). Compared to microarray assays, the new assay system makes the convenient, low cost and individualized subtype diagnosis of pediatric ALL a reality and is clinically applicable, particularly in developing countries.
منابع مشابه
Cost-analysis of Treatment of Pediatrics Acute Lymphoblastic Leukemia based on ALL-BFM Protocol
Background Among all pediatric cancers acute lymphoblastic leukemia (ALL) is highly prevalent, butthe overall cost of ALL management is not well-known, there is a need to assess the Berlin-Frankfurt-Munster (BFM) protocol commonly used in ALL management in Iran. So, the aim of this study was to estimate direct and indirect costs of ALL treatment among children based on the ALL-BFM protocol from...
متن کاملInterleukin-23 Receptor Gene Variants in Acute Lymphoblastic Leukemia and Their Relation to Prognostic Factors
Background: Interleukin (IL)-23 has an important role in tumor immune regulation. Objective: To investigate the possible association of interleukin-23 receptor (IL23R) gene variants rs1884444, rs10889677 and rs11209026 with development of acute lymphoblastic leukemia (ALL). Methods: The IL23R variants were studied in 164 ALL patients and compared to 175 healthy controls by polymerase chain reac...
متن کاملTreatment of Ifosfamide -induced Encephalopathy with Methylene Blue in an Adolescent with Relapsed Acute Lymphoblastic Leukemia: a case report
Ifosfamide is one of the most important chemotherapeutic agents used for the treatment of numerous types of malignancies. Neurotoxicity is one of its life-threatening complications and is potentially fatal. In this paper we present a case of severe ifosfamide- induced encephalopathy in an adolescent with acute lymphoblastic leukemia in whom treatment with methylene blue led to complete improvem...
متن کاملBone Density in Pediatric Patients with Acute Lymphoblastic Leukemia (ALL): A Literature Review
Introduction: Acute Lymphoblastic Leukemia (ALL) is the most common malignancy in children and the main form of childhood leukemia (75%). ALL different treatment options have a great impact on children weight and appetite. The improving prognosis for children with cancer refocuses attention to long-term outcomes with an emphasis on quality of life. More survival rate allows researchers to eval...
متن کامل